Tris (hydroxymethyl) aminoomethane

☑ Tris buffer abụghị naanị ejiri ya mee ihe dị ka ihe mgbaze maka acid nucleic na protein, mana nwekwara ọtụtụ ihe dị mkpa.Ejiri Tris maka uto kristal protein n'okpuru ọnọdụ pH dị iche iche.
☑ Enwere ike iji ike ionic dị ala nke Tris buffer mepụta eriri etiti nke lamin na C. elegans.
☑ Tris bụkwa otu n'ime ihe ndị mejupụtara protein electrophoresis buffer.
☑ Na mgbakwunye, Tris bụ onye etiti maka nkwadebe nke surfactants, vulcanization accelerators na ụfọdụ ọgwụ.A na-ejikwa Tris dị ka ọkọlọtọ titration.
☑A na-eji Trimethylaminomethane eme ihe na nnukwu metabolic acidemia na iku ume.Ọ bụ ihe nchekwa alkaline ma nwee mmetụta nchekwa dị mma na metabolic acidosis na mmeghachi omume ọrụ enzyme.
☑ A na-ejikarị Tris dị ka ihe nchekwa ndu, yana uru pH ya bụ 6.8, 7.4, 8.0, 8.8.Usoro nhazi ya, uru pH na-agbanwe nke ukwuu na okpomọkụ.N'ikwu okwu n'ozuzu, uru pH na-ebelata site na 0.03 mgbe okpomọkụ na-abawanye site na otu ogo.A na-eji Tris eme ihe n'ọtụtụ ebe n'ịkwadebe ngwọta nchekwa na biochemistry na nyocha ihe ọmụmụ ihe omimi.Dịka ọmụmaatụ, ma TAE na tbe buffers (nke a na-eji maka mgbasa nucleic acid) nke a na-ejikarị na nyocha biochemical chọrọ Tris.N'ihi na o nwere otu amino dị iche iche, ọ nwere ike imeghachi omume na aldehydes.
☑ Tris bụ ntọala adịghị ike, yana PKA bụ 8.1 na ọnụ ụlọ okpomọkụ (25 ℃).Dabere na tiori buffer, oke nchekwa nke Tris buffer dị n'etiti 7.0 na 9.2.Uru pH nke Tris base aqueous solution bụ ihe dịka 10.5.N'ozuzu, a na-agbakwunye hydrochloric acid iji mezie uru pH na uru achọrọ iji nweta ngwọta nchekwa na uru pH a.N'otu oge ahụ, anyị kwesịrị ịṅa ntị na mmetụta nke okpomọkụ na pKa nke Tris.N'ihi na ihe nchekwa Tris bụ ihe ngwọta alkaline na-adịghị ike, DNA ga-ewepụ ya na ngwọta dị otú ahụ iji melite solubility ya.Ndị mmadụ na-etinyekarị EDTA n'ime ihe nchekwa Tris hydrochloric acid iji mee "Te buffer", nke a na-eji maka nkwado DNA na nchekwa.Ọ bụrụ na ihe ngwọta acid nke imezi uru pH na-anọchi anya acetic acid, a na-enweta "Tae buffer" (Tris / acetate / EDTA), ma nweta "tbe buffer" (Tris / borate / EDTA) site na iji boric acid dochie ya. .A na-ejikarị ihe nchekwa abụọ a na-eme nchọpụta nucleic acid electrophoresis.